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Created with Raphaël 2.2.018Nov24Jun12112May113Apr1219May29Oct28119432125Sep23Add LICENSEmastermasterIncluded word doc with additional instructionsdeveldevelMerge branch 'improved-architecture'changed titlepdf with instructionssave full outputupdate readmedeleted old filessemicolons for table row setupsave normalized roi tracescomplete file for doing manual processing. select save file for all info csv and summary filefile for calculating the ROI means from the sequencefile for creating a mask of the rois from the .png made by manually labeling roiscreate mask for manual labelsfile for building mask of ROIs from manual methodFile for working with manually labeled image ROIschanged trailing backslash to filesep for cross platform functionalityfunction for median filtering image sequence and overlaying making overlay videomedian filter sequenceimproved instructionsinstructions for how to useupdates namesbetter messageadd gridnormalize to specific input baselineseperate file for loading the roi file and analyzingget input from the user for which frames to use as baseline and stimfunction to plot roi tracesfunction for getting roi centroidsfunction to wrap all roi detection into one place. output is binary roi imagefunction for creating output videonew file for overlaying roi imagefixed name errorfunction for calculating the thresholded absolute laplacianuse new laplacian filefurther updates to main filefunction for calculating laplacian of imagefile for getting brightest image and updated main filefile to get the trace for the mean brightness of each frame in sequencefunction for loading sequence of images